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KMID : 1007519950040040258
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Food Science and Biotechnology
1995 Volume.4 No. 4 p.258 ~ p.263
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Production and Characteristics of Foreign Glucoamylase from Recombinant Saccharomyces cerevisiae
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YOO YOUNG-JE
Cha Hyung-Joon
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Abstract
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The optimal medium components and composition for foreign glucoamylase production and the characteristics of cloned foreign glucoamylase were investigated for a recombinant yeast system. This system consisted of the yeast Saccharomyces cerevisiae transformed with the 2 μ-based recombinant plasmid YEpSUCSTA which contains the SUC2 promoter and glucoamylase coding STA gene including the signal sequence and structural gene. Large amounts of foreign glucoamylase were produced in a medium containing glucose and starch. It was thought that the recombinant yeast MMY2SUCSTA utilized starch as a carbon source for glucoamylase biosynthesis. The optimal concentrations of glucose and starch were both 10 g/L. Yeast extract was an important factor for foreign glucoamylase biosynthesis while the optimal concentration was 10 g/L. The optimal pH and temperature for the activity of purified glucoamylase were 5.0 and 60℃, respectively. Kinetic parameters V_(max) and K_m involved in the Michaelis-Menten equation were calculated as 0.509 g/L·min and 2.28 g/L, respectively.
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